You wrote: I'm wondering how to quench auto-fluorescence in tissue from a mouse that was treated with a FITC-labeled protein. What do people usually use to quench the endogenous auto-fluorescence, and will this treatment
quench the FITC signal from the labeled protein? Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 First, you didn't say how the tissues were treated after removal from animal? Are you doing FFPE or frozen sections, etc???? Was this an injected protein-FITC? More details will help. A method to remove auto-fluorescence will depend on the type of auto-fluorescence you experience due to the fact there are more than one source of this problem. An excellent discussion with methods for removal are found on the Wright Cell Imaging Facility website. http://www.uhnres.utoronto.ca/facilities/wcif/PDF/Autofluorescence.pdf Good luck Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT 59715 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet