I am in need of some help with Hif-1 alpha that was purchased through Novus. 
The tissue I am working with is mouse breast cancer and mouse lung cancer and 
the tissue is frozen and fixed in acetone for 10 mins. I am not getting any 
specific staining because my Negative Control looks just like my Positive 
Control tissue. I have tried no treatment, Protease 2, pH 8.4 with heat and no 
heat through the process on a Ventana Discovery XT.  I need some one's protocol 
or recommendations as to how I should proceed with additional optimizing. 
Thanks!!

John
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