We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184.
1. Prepare the following aqeous stock solutions: a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter) b. 2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter) 2. Dilute DAB solution of necessary so that its concentration does not exceed 0.9 mg/ml. 3. For each 10ml of DAB solution, add: a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter) b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%) 4. Allow mixture to stand for at least 10 hours. It is now non-mutagenic. 5. Decolorize the mixture with ascorbic acid (add powder until color disappears). This too is an oxidation/reduction reaction. 6. Neutralize the decolorized mixture with sodium bicarbonate (test with pH meter, pH paper or dipsticks). Note: we recommend using magnesium hydroxide/oxide instead of sodium bicarbonate. 7. Discard the solution down the drain provided that local wastewater authorities have given their approval. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Sherwood, Margaret " <msherw...@partners.org> 06/17/09 9:30 AM >>> We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet