Protocol #3 at this site is great for RNA probes:
http://intramural.nimh.nih.gov/lcmr/snge/Protocols/ISHH/ISHH.html
This protocol assumes fresh frozen tissue but can be used on fixed, paraffin
embedded tissue--obviously you would have to include a step at the beginning
to deparaffinize. You might also want to look at HIER methods that can
improve results from fixed tissue.
----- Original Message -----
From: "Bell, Pat" <pat.b...@ucdenver.edu>
To: <histonet@lists.utsouthwestern.edu>
Sent: Wednesday, June 17, 2009 12:35 PM
Subject: [Histonet] RNA In Situ Hybridization
I want to learn to do In Situ Hybridization using RNA probes in formalin
fixed, paraffin embedded tissues. I have the Dako Autostainer, but I know
that some of the procedure would be done manually.
I would appreciate any protocols that would help me.
Thank you for your help.
Pat
Pat Bell HT(ASCP)
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu<mailto:pat.b...@ucdenver.edu>
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