From: <histonet-requ...@lists.utsouthwestern.edu> Reply-To: <histonet@lists.utsouthwestern.edu> Date: Thu, 18 Jun 2009 03:12:15 +1000 To: <histonet@lists.utsouthwestern.edu> Conversation: Histonet Digest, Vol 67, Issue 17 Subject: Histonet Digest, Vol 67, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. PR and ER staining in dog uterus (Karen Doty) 2. Re: Inactivation of DAB (Sherwood, Margaret ) 3. RE: Research Genetics (Reynolds,Donna M) 4. Looking for NYU contact (Angela Bitting) 5. Re: Re: Inactivation of DAB (Lynette Pavelich) 6. RE: Re: Inactivation of DAB (CHRISTIE GOWAN) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Jun 2009 15:10:06 -0500 From: Karen Doty <k...@live.com> Subject: [Histonet] PR and ER staining in dog uterus To: <histonet@lists.utsouthwestern.edu> Message-ID: <col104-w3115715881a9aeb048fa6ce4...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi, Folks, We are starting to work on a project that requires PR and ER staining in dog uterus. I'm hoping someone will share some tips such as which antibodies work well in dog (clone, manufacturer), and any specific special procedures that might help, such as which antigen retrieval methods work best. I've tried a couple antibodies already without good results in dog although they worked very well in a mouse control. All suggestions and advice is appreciated. Thanks very much. Karen Doty Dept. of Veterinary Biosciences University of Illinois _________________________________________________________________ Insert movie times and more without leaving HotmailĀ®. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009 ------------------------------ Message: 2 Date: Wed, 17 Jun 2009 09:30:55 -0400 From: "Sherwood, Margaret " <msherw...@partners.org> Subject: [Histonet] Re: Inactivation of DAB To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <073ae2bea1c2ba4a8837ab6c4b943d9703e23...@phsxmb30.partners.org> Content-Type: text/plain; charset="us-ascii" We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 3 Date: Wed, 17 Jun 2009 08:33:15 -0500 From: "Reynolds,Donna M" <dreyn...@mdanderson.org> Subject: [Histonet] RE: Research Genetics To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <785bbf0c5f49ce41ba74460a43a08f021449221...@dcpwvmbxc0vs3.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Research Genetics has merged a couple of time the last few years. They are now Open Biosystems in Huntsville, Al pho 888 412 2225. They do not list the Research Genetics on the Web but last I knew products were still available. You can call them to get current catalog number and prices. Donna Reynolds HT (ASCP) Chief Histology Laboratory U. T. M.D. Cancer Center Cancer Biology Core Immunohisto Lab 713-792-8106 Message: 2 Date: Mon, 15 Jun 2009 14:52:39 -0400 From: "Joanne Mauger" <mau...@email.chop.edu> Subject: [Histonet] Re: research genetics/phoenix biotech To: <jluis.pala...@icman.csic.es>,<histonet@lists.utsouthwestern.edu> Message-ID: <4a3660370200003100007...@email.chop.edu> Content-Type: text/plain; charset=US-ASCII Hi everyone, I am unable to find this company anymore. Does anyone know if old research genetics products are still being sold? I don't want Invitrogen- they did away with the product I want-MicroHyb. Thanks, Jo Mauger ------------------------------ **** ------------------------------ Message: 4 Date: Wed, 17 Jun 2009 09:50:09 -0400 From: "Angela Bitting" <akbitt...@geisinger.edu> Subject: [Histonet] Looking for NYU contact To: <histonet@lists.utsouthwestern.edu> Message-ID: <4a38bc51.2b7f.00c...@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Just wondering if anyone can give me contact information for someone in the Neuropath Lab at NYU? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 5 Date: Wed, 17 Jun 2009 10:05:13 -0400 From: "Lynette Pavelich" <lpave...@hurleymc.com> Subject: Re: [Histonet] Re: Inactivation of DAB To: <histonet@lists.utsouthwestern.edu>,<msherw...@partners.org> Message-ID: <4a38bfd9020000ee0002a...@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184. 1. Prepare the following aqeous stock solutions: a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter) b. 2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter) 2. Dilute DAB solution of necessary so that its concentration does not exceed 0.9 mg/ml. 3. For each 10ml of DAB solution, add: a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter) b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%) 4. Allow mixture to stand for at least 10 hours. It is now non-mutagenic. 5. Decolorize the mixture with ascorbic acid (add powder until color disappears). This too is an oxidation/reduction reaction. 6. Neutralize the decolorized mixture with sodium bicarbonate (test with pH meter, pH paper or dipsticks). Note: we recommend using magnesium hydroxide/oxide instead of sodium bicarbonate. 7. Discard the solution down the drain provided that local wastewater authorities have given their approval. Hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: lpave...@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Sherwood, Margaret " <msherw...@partners.org> 06/17/09 9:30 AM >>> We have started doing routine immunohistochemistry on our stainer and have a large waste container that includes diaminobenzidine (DAB). We would like to inactivate it so we don't have to pay to have the waste removed. Can anyone help me? What do people use to inactivate their DAB? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 17 Jun 2009 14:27:39 +0000 From: CHRISTIE GOWAN <christiego...@msn.com> Subject: RE: [Histonet] Re: Inactivation of DAB To: <msherw...@partners.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <snt110-w259f909487498cdb4f48a8ae...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi Peggy, You wrote: > We have started doing routine immunohistochemistry on our stainer and have a > large waste container that includes diaminobenzidine (DAB). > > We would like to inactivate it so we don't have to pay to have the waste > removed. Can anyone help me? What do people use to inactivate their DAB? May I suggest that you access the histonet archives where you will be able to see all the previous discussions on this topic. The responses were many and varied depending on different lab types and locations. Good luck with your search. Respectfully, Christie Gowan Surgical Pathology University of Alabama Hospital ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 67, Issue 17 **************************************** No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.339 / Virus Database: 270.12.71/2178 - Release Date: 06/16/09 21:23:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet