Hi everyone,  
Has anyone had problems with TTF-1 not staining Bouin's fixed tissue.  We stain 
ours on the DAKO Autostainer with Envision+ Dual Link HRP System.  We use DAKO 
pH9 target retrieval in the pressure cooker, and use a 1:50 dilution of DAKO 
TTF-1 (M3575).   
   
Ellen Yee  
Diagnostic Pathology Medical Group, Inc.  
Sacramento, CA
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