Good old fashioned gelatin subbing is very cheap & works great--I have never
seen tissue separate from a subbed slide! Susan
----- Original Message -----
From: "Peggy Bisher" <mbis...@princeton.edu>
To: "Milne, Katy" <kmi...@bccancer.bc.ca>;
<histonet@lists.utsouthwestern.edu>
Sent: Friday, June 19, 2009 3:34 PM
Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus
I had a group here who were having trouble with their cryo-sections
sticking
to the slides. Their samples were rat brains. He found that the Gold Super
Frost Plus worked well with in-situ but not for immuno. At least that is
the
comment he wrote down and put on our bulletin board in the lab. Most of
the
people in the lab tend to use the Super Frost Plus (not the Gold) and they
seem pretty happy with them.
Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbis...@princeton.edu
On 6/19/09 4:26 PM, "Milne, Katy" <kmi...@bccancer.bc.ca> wrote:
Hi everyone,
I'm curious if anyone has tried using Superfrost plus gold slides, are
they any better than the regular superfrost plus? I'm dealing with
frozen lymph node on a Ventana discovery, I've tried a few different
fixing/drying combinations but I still have tissue lifting (not
completely but enough to be bothersome!). Wondering if it's worth
giving the gold slides a go.
Thanks,
Katy
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