Lynn
From: lynn...@hotmail.com
To: lynn...@hotmail.com
Subject: RE: [Histonet] xylene and frozen sections
Date: Thu, 18 Jun 2009 19:53:52 -0500
Is there any literature that says xylene will fade progressive staining H&E
(using Surgipath Harris Hematoxylin) over a period of years? Our frozen
staining series after the eosin has two 95% ROH's and six 100% and then clear
in Propar. I mistakenly replaced the last three 100% ROH with xylene when I
rotated the series. I'm told that anyone who knows anything about chemistry
knows this is a big deal and the sections will fade. Every lab I've worked in
used xylene to clear frozen sections. I understand that I messed up because
this isn't our proceedure, but I don't believe I sacrificed patient care. Am I
wrong?
Lynn
> From: lynn...@hotmail.com
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 18 Jun 2009 19:47:14 -0500
> Subject: [Histonet] xylene and frozen sections
>
>
>
>
>
> Lynn
>
>
> _________________________________________________________________
> Insert movie times and more without leaving Hotmail®.
> http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutorial_QuickAdd_062009
Insert movie times and more without leaving Hotmail®. See how.
_________________________________________________________________
Hotmail® has ever-growing storage! Don’t worry about storage limits.
http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet