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Today's Topics:
1. Re: Beta-Amyloid (Richard Cartun)
2. Millipore MAB1281 anti human nuclei IHC on FFPE
(PALMER Jason (SVHM))
3. Re: Frozen Section TAT (Phyllis Thaxton)
4. Signs of good perfusion (Thach, Dzung (NIH/NIAID) [E])
5. Cardboard Paraffin Catchers (Phyllis Thaxton)
6. Re: Signs of good perfusion (Merced M Leiker)
7. WI/MI (Steven Coakley)
----------------------------------------------------------------------
Message: 1
Date: Sun, 28 Jun 2009 20:47:27 -0400
From: "Richard Cartun" <rcar...@harthosp.org>
Subject: Re: [Histonet] Beta-Amyloid
To: "Histonet" <histo...@pathology.swmed.edu>, "Sebree Linda A"
<lseb...@uwhealth.org>
Message-ID: <4a47d6df.7400.007...@harthosp.org>
Content-Type: text/plain; charset=US-ASCII
We've been using a monoclonal antibody (clone 6E10) from Signet
Laboratories (now Invitrogen) for years with excellent results.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
"Sebree Linda A" <lseb...@uwhealth.org> 6/24/2009 2:49 PM >>>
Hi,
Wondering if anyone knows of a Beta-Amyloid antibody, for use on FFPE
brain sections, that doesn't need formic acid pretreatment?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
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------------------------------
Message: 2
Date: Mon, 29 Jun 2009 16:36:12 +1000
From: "PALMER Jason (SVHM)" <jason.pal...@svhm.org.au>
Subject: [Histonet] Millipore MAB1281 anti human nuclei IHC on FFPE
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<eee122d54674bf4ca53c0d05f9bb939e04d56...@svhm-exchccr0.svhm.schs.org.au>
Content-Type: text/plain; charset="us-ascii"
Hi all.
Just wondering if anyone has managed to use Millipore mouse anti human
nuclei antibody (clone 235-1, MAB1281) successfully with immunostaining of
FFPE tissues?? The datasheet says it is possible, at 1:20 and with
citrate retrieval but I have had no luck with this on a variety of human
tissues, using a standard ABC with DAB protocol. Also no luck with
proteinase K. (There is also an IHC world protocol which uses 1:20 with
citrate.) I see some apparent labelling of epidermal nuclei, for example,
maybe half of all nuclei , but also see the same thing in diluent-only
negative controls, so clearly the staining seen is not specific.
Thanks for any help,
Jason
Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.pal...@svhm.org.au
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Message: 3
Date: Mon, 29 Jun 2009 06:58:21 -0700 (PDT)
From: Phyllis Thaxton <dch...@yahoo.com>
Subject: Re: [Histonet] Frozen Section TAT
To: "Lott, Robert" <robert.l...@trinitymedicalonline.com>,
histonet@lists.utsouthwestern.edu
Message-ID: <327546.90788...@web43502.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All,
I haven't been on in a couple of weeks, but thank all of you for the
discussion on FS turnaround time reporting. I thought we had everything in
place, but one thing we didn't even consider is when breast biopsies are
send for radiology before arriving for FS.
We are also reporting receiving time as order time because they come in a
pneumatic zip tube in -60 seconds. I guess if there is more than one room
trying to send and are in line waiting to be zipped, then the order time
would be inaccurate the way we are reporting it.
Thanks all!!
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
________________________________
From: "Lott, Robert" <robert.l...@trinitymedicalonline.com>
To: histonet@lists.utsouthwestern.edu
Sent: Friday, June 26, 2009 11:00:27 AM
Subject: [Histonet] Frozen Section TAT
Becky Garrison makes some very valid and interesting points below....
First of all, the point about what a "critical test" is, as defined by
JCHAO.
She is correct, in that these are tests defined by the individual
institution in policy, but not by JCAHO.
Second, the item (ANP.11820) on the CAP checklist concerning turnaround
time of intraoperative frozen sections reads as follows:
The institution must "periodically" evaluate TAT and document reasons
for delay BUT only if 90% of FS are not completed within 20 minutes.
ANP.11820 Phase 1
Does the laboratory periodically evaluate turnaround time for
intraoperative frozen sections?
NOTE: If 90% of frozen sections are not completed within 20 minutes,
the laboratory must document evaluation
of the reason(s) for the delay. This turnaround time is intended to
apply to the typical single frozen section.
In cases where there are multiple sequential frozen sections required on
a single specimen (e.g., resection margins),
or in cases where additional studies such as radiographic correlation
are required, longer turnaround times may be expected.
COMMENTARY:
N/A
REFERENCE:
Novis DA, Zarbo RJ. Interinstitutional comparison of frozen section
turnaround time.
A College of American Pathologists Q-Probes study of 32,868 frozen
sections in 700 hospitals.
Arch Pathol Lab Med. 1997;121:559-567.
Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology
Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL
35213/ 205-592-5387
Message: 19
Date: Thu, 25 Jun 2009 10:49:43 -0400
From: "Garrison, Becky" <becky.garri...@jax.ufl.edu>
Subject: RE: [Histonet] Re: tracking turnaround time of
intraoperativeconsultations
To: "Della Speranza, Vinnie" <del...@musc.edu>, "Robert Richmond"
<rsrichm...@gmail.com>, <histonet@lists.utsouthwestern.edu>
Message-ID:
<b3ca1baf6c5e4541867e4e79ad2412e003c29...@jaxmail.umc.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Sorry I did not respond yesterday. The reason we just started measuring
order to sign out for frozens was also prompted by preparation for our
next JCAHO inspection. However, there is this distinction. The Joint
Commission does not define a frozen as a critical test. The designation
of critical test is left up to the individual institution. However,
once your institution defines the frozen as a critical test, (indicated
somewhere in a policy), you must conform to JCAHO guidelines for
critical tests.
And apparently this is the buzz with JCAHO watchers right now.
Here we designate the IntraOp consultations for frozens (not gross only
or Touch prep) and IntraOp PTH (Clinical test) as a critical tests
and have started tracking order to sign out. Order time is the time the
surgeon indicates 'send this to pathology' not when pathology receives
the specimen.
We are somewhat in uncharted waters as there is no national standard
that defines target time from order to sign out. We set a 40 minute
time (20-OR to Path and 20-path to completed frozen). I campaigned
against a 30 minute total time (15 each) because we do have some frozens
that do take over 15 minutes and this was an absolute value (unlike the
CAP goal of 90% within 20 minutes). Our approach is to monitor,
evaluate the data we retrieve. There will certainly be adjustments made
to target time and how and what we monitor.
The data collection raises more questions: how do you come up with
meaningful data for multiple specimens on a single case; multiple
frozens (different patients) received together or before we are finished
with the first patient's frozen). This is one of those ideas that
sounds good in theory but presents some challenges in execution. But it
is a valid process to monitor as we periodically have surgeons complain
of the time they are
waiting for frozen results. This is really a joint quality management
review which involves multiple departments (OR and Pathology) and how we
make it better for the patient.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237
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------------------------------
Message: 4
Date: Mon, 29 Jun 2009 11:31:25 -0400
From: "Thach, Dzung (NIH/NIAID) [E]" <thac...@niaid.nih.gov>
Subject: [Histonet] Signs of good perfusion
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID: <c66e568d.d7d%thac...@niaid.nih.gov>
Content-Type: text/plain; charset="iso-8859-1"
Hi Everyone!!
I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am
nicking the upper right atrium of heart to collect the gushed out blood
and
then perfusing through ventricle using a 21G butterfly needle and
peristaltic pump. Sometimes I see the lungs swelling up and fluid comes
out
of mouth. Occasionally, I see the liver fade to light pink. Most of the
time the paws become white. But the brain and spinal cord (my tissues of
interest) are always white, and seem to have been perfused. I was
wondering
how to improve this to get more consistent good perfusions, and what signs
should I look for to indicate good perfusion?
Thanks much,
Dzung
NIAID
------------------------------
Message: 5
Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT)
From: Phyllis Thaxton <dch...@yahoo.com>
Subject: [Histonet] Cardboard Paraffin Catchers
To: Histonet@lists.utsouthwestern.edu
Message-ID: <372922.65853...@web43506.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone remember the little cardboard microtome trays for paraffin
waste? If so please email me the company that makes them.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
------------------------------
Message: 6
Date: Mon, 29 Jun 2009 12:07:04 -0400
From: Merced M Leiker <lei...@buffalo.edu>
Subject: Re: [Histonet] Signs of good perfusion
To: "Thach, Dzung (NIH/NIAID) [E]" <thac...@niaid.nih.gov>,
histonet@lists.utsouthwestern.edu
Message-ID: <1d8e958050abad54bd852...@cdywxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
Live going pale is a good sign, your tissues of interest going pale is an
even better sign, but fluid coming out of the mouth (or even the nose, or
additionally, any kind of bloating or swelling in the animal) is a bad
sign. You may not be able to get good perfusion (pale tissues) if this
happens before your tissues turn pale, as the pressure is too high causing
fluid to leak out of the vasculature....ideally you want to push the blood
out through the the hole you made in the right atrium, not through the
walls of the vessels. at what rate do you perfuse? if this happens a lot
slow it down.
Hope this helps.
--On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]"
<thac...@niaid.nih.gov> wrote:
Hi Everyone!!
I am perfusing CO2 euthanized 3 weeks old mice with PBS only. I am
nicking the upper right atrium of heart to collect the gushed out blood
and then perfusing through ventricle using a 21G butterfly needle and
peristaltic pump. Sometimes I see the lungs swelling up and fluid comes
out of mouth. Occasionally, I see the liver fade to light pink. Most of
the time the paws become white. But the brain and spinal cord (my
tissues of interest) are always white, and seem to have been perfused. I
was wondering how to improve this to get more consistent good perfusions,
and what signs should I look for to indicate good perfusion?
Thanks much,
Dzung
NIAID
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Histonet@lists.utsouthwestern.edu
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 7
Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT)
From: Steven Coakley <sjchta...@yahoo.com>
Subject: [Histonet] WI/MI
To: Histonet@lists.utsouthwestern.edu
Message-ID: <385293.93112...@web38204.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I'm looking for a few techs from Northern WI or Northern MI to let me know
when HT positions become availiable.
Thanks
------------------------------
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End of Histonet Digest, Vol 67, Issue 32
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