We use eosin on the gross bench. We keep a bottle of concentrated Eosin and Hematoxylin on the bench with an eyedropper. The eosin is for endoscopic biopsies and the hematoxylin is for prostate biopsies. Neither has never interfered with IHC staining.
Debbie M. Boyd, HT(ASCP),Chief Histologist, Southside Regional Medical Center, 200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F: 804-765-5582, [email protected] Jennifer Johnson <[email protected]> Sent by: [email protected] 07/01/2009 01:09 PM To <[email protected]> cc Subject [Histonet] Re: Eosin in alcohol We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Hotmail® has ever-growing storage! Don?t worry about storage limits. http://windowslive.com/Tutorial/Hotmail/Storage?ocid=TXT_TAGLM_WL_HM_Tutorial_Storage_062009_______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
