My recommendation to you is to focus on your undemineralized bone
sections embedded in MMA. These sections will provide you with the
most information.
If you do a Goldners stain you can very accurately measure BV/TV and
clearly quantitate osteoid volume. Bone will stain green from the SF
yellowish staining and osteoid red with the acid fuchsin/ponceau. I
feel the only limitation with this stain is clearly assessing your
bone cells.
If you stain with a Von Kossa/MacNeal's tetrachrome you can achieve
the above (bone = black, osteoid = greyish-green) and you can also
clearly assess osteoblast (blue) activity. This stain also helps to
identify osteoclast presence but hard to accurately determine activity
without a TRAP stain follow up.
Write me back with any additional questions you may have and when I
get to the lab I will forward you a couple of protocols and additional
information!
Best,
Jack
Sent from my iPhone
On Jul 2, 2009, at 7:59 AM, "Manav Mehta" <manav.me...@charite.de>
wrote:
Dear Histonet'ers,
I am trying to stain for Osteoids in a fracture callus of rat
femurs. I
have MMA and Paraffin sections (4-5 um) / blocks already made.
However,
I am not sure what stain I should use. Peers have suggested that a
Movat
pentachrome should work, while some have suggested Goldner, and silver
staining. I am not sure how to proceed at the moment. I would
appreciate
any suggestions with possibly a protocol on the staining for osteoids.
It would also help if you provided references on this stain or details
on the stain. Life experiences while working with these stains are
also
welcome.
The Goal is to characterize the osteoids in the callus (surface,
thickness, osteoblast numbers). Possibly relate them to osteoblastic
and
-clastic activity.
Thanks,
Manav Mehta
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