I agree...... Also you're not storing them in sunlight are you? Silly question I know.
Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 02 July 2009 20:28 To: histonet@lists.utsouthwestern.edu; sr...@aol.com Subject: Re: [Histonet] Help With Hemo Fading The fading most probably is caused by acid in the permanent slide, probably because the sections were passed through the alcohols very quickly after the acid differentiation, or they stayed little time in tap water after differentiation or no bluing agent was used. It is unlikely that the mounting medium is acidic, although that could also be the cause also. An acid environment over the cover slipped section is the most probable "culprit" for the henatoxylin fading. Check the staining protocol. René J. --- On Thu, 7/2/09, sr...@aol.com <sr...@aol.com> wrote: From: sr...@aol.com <sr...@aol.com> Subject: [Histonet] Help With Hemo Fading To: histonet@lists.utsouthwestern.edu Date: Thursday, July 2, 2009, 2:42 PM Hello everyone, ? We are having problems with short-term hematoxylin fading and loss of detail. The pathologist is freaking out! I've seen hemo fade over a long period of time but not in a matter of a few months. Slides from one year ago are really bad. ? I've been out of the business for a number of years and in the interim much has changed including reagents. These are GI tract biopsies processed by microwave. ? Any thoughts at all? ? Thanks! Marg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
