Hi Patti, Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a Hematopathologist at NIH. This formula was particularly great for BM Core BX's. It is much gentler on the tissue, and is less likely to harm the cellular integrity. Dr. Todd Barry also prefers the use of EDTA formula's for BM Bx's.
Below is the formula. or you can purchase from Biocare Medical. You should decal the specimen for 30 minutes to 1 hour and check for decalcification. DO NOT leave for any longer than 1 hour. 10% EDTA (Ethyl-media minetetra-acetic-acid Stock EDTA 10 ml DI water 90 ml pH 4.5-5.0 with Sodium Hydroxide If you prefer to purchase the IED Solution, Biocare Medical has it available. They have 2 sizes 140 ml or 500 ml. cat # IED 1204. Ion-Exchange Decalcification Unit (IED Unit, Designed for Bone Marrow Biopsies) An advanced decalcification system that removes calcium from bone quickly while leaving superior cellular detail. The IED Unit incorporates a strong cation ion-exchange resin in a weak acid solution to remove calcium ions from bone, replacing them with hydrogen ions. Because the IED Unit does not require strong concentrated acid solutions, as in traditional decalcification methods, delicate cellular structures antigenicity remain intact. Regards, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 E-Mail: aallison-ta...@apmglab.com --- On Fri, 7/10/09, Patti Loykasek <ployka...@phenopath.com> wrote: From: Patti Loykasek <ployka...@phenopath.com> Subject: [Histonet] Bone marrows To: "histonet" <histo...@pathology.swmed.edu> Date: Friday, July 10, 2009, 12:38 PM Hi All. I was wondering what everyone's current favorite bone marrow decal solution/method is. Including commercially available decal solutions. We will be doing IHC after fixation/decal. Thanks for the input (as always). Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet