Igor.
A few years back I used Dako V9 mouse anti human vimentin to label human grafts
in a mouse background. Tested it first on several mouse tissues and got no
reactivity, compared to very strong reactivity in a variety of cell types in
human tissue, and so am sure that it is human specific cf mouse. I used the
Dako ARK to get around the mouse-on-mouse background issues and was happy with
the staining obtained (although not quite as sensitive perhaps as a standard,
LSAB method). I am actually about to try this again myself very soon. I used
citrate AR and primary at 1:800 for my staining.
Cheers,
Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.pal...@svhm.org.au
------------------------------
Message: 4
Date: Tue, 14 Jul 2009 15:03:47 -0400
From: Igor Deyneko <igor.deyn...@gmail.com>
Subject: [Histonet] Human VIMENTIN and SMA IHC
To: Histonet@lists.utsouthwestern.edu
Message-ID:
<35e16a770907141203h14ccc18bt2e3123d11c478...@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear Histonetters!
I am wondering if anyone can possibly advise good antibodies for HUMAN anti
alpha SMA and Vimentin. I'm working with xenografts, human tumors with mouse
stroma and in the past had a lot of cross reactivity and background issues.
Does anyone know good antibodies or a clone, or has a good protocol for
either???
All would be greatly appreciated.
Thank you.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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