HI: I have a = question regarding keeping livers intact. I am currently removing o= rgans of dosed animals and freezing them in OCT. I cryostat these livers = in 40um sections and are kept in -80C until used. We fix thes= e using 4% Paraformaldehyde, and we use antibodies and DAB for histochemist= ry. What I have been seeing is that my tissue almost looks expanded. Sm= all tissue gaps are magnified and it looks as if chunks of the tissue just = dissapear leaving gaps in between what looks like cell clusters. Th= is is not there when I cut or during -80 storage because I check the slides before and after freezin, and fixation. I would greatly appreciate= some input on how to prevent this from happening. Tha= nks Sean Taheri = This e-mail and any attachment hereto, is intended= only for use by the addressee(s) named above and may contain legally privi leged and/or confidential information. If you are not the intended recipi= ent of this e-mail, any dissemination, distribution or copying of this emai= l, or any attachment hereto, is strictly prohibited. If you receive this = email in error please immediately notify me by return electronic mail and p= ermanently delete this email and any attachment hereto, any copy of this e-= mail and of any such attachment, and any printout thereof. Finally, pleas= e note that only authorized representatives of Regeneron Pharmaceuticals, I= nc. have the power and authority to enter into business dealings with any third party. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
