You can use periodic acid to oxidize your tissues as you would for the PAMS
stain. However, this is not the optimal solution when staining fungi. 5%
chromium trioxide used for the GMS can be reused. Since you are doing the
stains manually, as many of us do, this works particularly well. The fresh
chromium trioxide is a clear orange color. It can be reused until the color
turns more brown in color. This indicates that the oxidizing potential has
been depleted. You can also use 3% chromium trioxide instead of the 5% and
heat the solution to 55 degrees and oxidize for 30 minutes. Finally you can
use 2% chromic acid in the microwave oven at 70% power for 45 seconds
(solution should be about 55 degrees). By using milder solutions or reusing
your solution you will save money and time.
Cheryl
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