Yes, with H&E staining. They think they need a thick section because
they are looking for product that is 75mm round. I don't seem to be
able to make them understand that it is not necessary to cut that thick
to see the beads they are looking for.
*/--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-534-4655 voice
858-534-5304 fax
CONFIDENTIALITY NOTICE:
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PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL
AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR
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Barbara Albert wrote:
Karen,
Are you certain that they want these sections on slides? The thicker
ones we usually put into a microcentrifuge tube. No water bath, and
the curled section fits into the tube.
Barbara Albert
San Francisco
------------------------------------------------------------------------
*From:* kbowden <kbow...@ucsd.edu>
*Cc:* histonet <histonet@lists.utsouthwestern.edu>
*Sent:* Wednesday, July 22, 2009 4:46:31 PM
*Subject:* [Histonet] 100 micron sections
Hi,
I am being asked to section paraffin embedded fat at 100 microns. I
haven't sectioned anything in paraffin thicker than 20 microns. What
are the tricks to get it to unroll in the water bath? -- */--
Karen Bowden
Staff Research Associate II
University of CA, San Diego
Department of Orthopedics
9500 Gilman Dr. 0630
La Jolla, CA 92093-0630
858-534-4655 voice
858-534-5304 fax
CONFIDENTIALITY NOTICE:
THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE
PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL
AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR
OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION
BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.
IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND
DELETE THE MATERIAL FROM ANY COMPUTER./*
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