We had a series of PIN 4 cocktail immuno stains and got a poor reaction.
I am afraid to cut deeper in the block and miss the area of concern.
I have determined the problem was due to the heat bar not being turned
on.
 
What can I do the slides to reverse the process and restain them.  The
DAB stained good, it is the vulcan fast red that was not reacting.
 
Diana 
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