We soak our nails in 20% sodium hydroxide for at least an hour, rinse in
running tap water for a few minutes before we place the cassettes on the
tissue processor. We use positive charged slides, heat slides in 80 degree
oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a lot
more gentler on the tissue that the GMS. We are the toenail lab for the U.S.
Air Force ,
Joe "The Toe"
----- Original Message -----
From: "jstaruk" <jsta...@masshistology.com>
To: "'Clare Thornton'" <cthorn...@dahlchase.com>;
<histonet@lists.utsouthwestern.edu>
Sent: Friday, July 24, 2009 10:41 AM
Subject: RE: [Histonet] GMS-Fungus on nails
Prepare a 10% solution of Titebond II premium wood glue (found in most
hardware stores). Dip the slide in the solution just before mounting the
section on the slide. Let the slide dry and stain away.
Jim
_______________________
James E. Staruk HT(ASCP)
www.masshistology.com
www.nehorselabs.com
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Friday, July 24, 2009 11:31 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] GMS-Fungus on nails
We do a GMS for fungus on all nails we receive. We often have a difficult
time keeping the nail tissue on the slide. We've tried baking for long
periods, pre-treating in formalin, using silane slides, with no luck.
Even
when the nails cut relatively easily we still lose it, and end up running
several GMS stains before we might get a speck or two of tissue we can
look
at. We use the Artisan stainer for our specials. We are really not
interested in performing the GMS manually, due to volume and turn around
time restrictions. Any suggestions?
Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com
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