I used to cut entire eyes of fish and lizards embedded in paraffin with great results. The only thing is that on those (many years ago) days I used clearing with Canada balsam followed by a short wash in benzene (not very safe) before the paraffin infiltration. René J.
--- On Wed, 7/29/09, Jo-Ann Bader, Ms. <jo-ann.ba...@mcgill.ca> wrote: From: Jo-Ann Bader, Ms. <jo-ann.ba...@mcgill.ca> Subject: [Histonet] Mouse Eyes To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, July 29, 2009, 11:38 AM We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada H3G 1Y6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
