Sharon, We process quite a few samples as well and I did them at Hopkins with a similar time frame for the old tissue. We did not experience the problems you are referencing when we pulled the cases out from storage. What is the exact type of artifact that you are seeing? Is it freeze/thawing artifact or is it the tiny holes that come from water crystals? I could send you our freezing technique if you like. I would also suggest using cryo vials that have rubber gaskets which are designed for use in those extreme temperatures. Walter Benton Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508
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