We do everything you do, except we surround the tissue with oct in the tube before freezing. Otherwise, it's freezer burn city. (in TX I think) :) We also just write the info on the side of the bottle with a thin sharpie instead of the cork thing. We usually store several tubes from the same case as we don't know how much tissue they will want in the future. Claire
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sharon Allen Sent: Wed 7/29/2009 9:27 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Artifact in Muscle bx's frozen for extended time Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic & research purposes & find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
