You running particularly small specimens like derm? You may need to decrease
time in alcohol. Adding a little ammonia water to your ice bath and soaking
after facing the block may help too.
------Original Message------
From: Kathleen Roberts
Sender: histonet-boun...@lists.utsouthwestern.edu
To: Nagappan, Peri
Cc: Histonet
Subject: Re: [Histonet] Paraffin Sections
Sent: Aug 4, 2009 1:40 PM
Peri,
Sounds like poor infiltration to me. What kind of tissue are we talking
about, and what processing program did you use?
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Rd
Piscataway, NJ 08854
Nagappan, Peri wrote:
>Hi Histonetters,
>
>
>
>When I cut the paraffin sections in the microtome, I am not getting the whole
>sections intact, rather some portion in the middle of the sections are
>brittle. But the paraffin portion surrounds the tissue is smooth, nice and
>intact.
>
>
>
>Thanks for your suggestion and help.
>
>
>
>Peri
>
>pnagap...@cau.edu <mailto:pnagap...@cau.edu>
>
><mailto:pnagap...@cau.edu>
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>
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