My answer is in the attached article I just published. Please read the d-Limonene discussion seciton. René J.
--- On Wed, 8/5/09, Sherwood, Margaret <msherw...@partners.org> wrote: From: Sherwood, Margaret <msherw...@partners.org> Subject: RE: [Histonet] Clear-Rite 3 To: "Va Paula Sicurello" <vapat...@yahoo.com>, "Rene J Buesa" <rjbu...@yahoo.com>, "HistoNet" <histonet@lists.utsouthwestern.edu> Date: Wednesday, August 5, 2009, 1:23 PM We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also would be curious you (and others) do not like d-limonene. Peggy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Wednesday, August 05, 2009 12:49 PM To: Rene J Buesa; HistoNet Subject: Re: [Histonet] Clear-Rite 3 Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine. I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. Without proper ventilation the safety people willnot allow the use of Xylenen. I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa <rjbu...@yahoo.com> wrote: From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, "BillO'Donnell" <billodonn...@catholichealth.net> Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all "pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. René J. --- On Wed, 8/5/09, O'Donnell, Bill <billodonn...@catholichealth.net> wrote: From: O'Donnell, Bill <billodonn...@catholichealth.net> Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet