Hi Patricia, I use an antibody from Spring Biosciences Cat#M3384 to stain our human xenograft tumors as well as human tissue. Antibody will also stain mouse tissue. Very nice clean crisp stain. At least when we use it on our Bond IHC stainiers. Thanks and let me know if you need more information. dusko
________________________________ From: "Zerfas, Patricia (NIH/OD/ORS) [E]" <zerf...@ors.od.nih.gov> To: dusko trajkovic <dunat...@sbcglobal.net> Sent: Monday, August 10, 2009 12:07:29 PM Subject: RE: [Histonet] CD31 working protocol Dusko, I am using mouse tissue. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ________________________________ From:dusko trajkovic [mailto:dunat...@sbcglobal.net] Sent: Monday, August 10, 2009 3:06 PM To: Zerfas, Patricia (NIH/OD/ORS) [E] Subject: Re: [Histonet] CD31 working protocol What species are you trying to stain? thanks Dusko ________________________________ From:"Zerfas, Patricia (NIH/OD/ORS) [E]" <zerf...@ors.od.nih.gov> To: " histonet@lists.utsouthwestern.edu " < histonet@lists.utsouthwestern.edu > Sent: Monday, August 10, 2009 11:50:50 AM Subject: [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda , MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet