I always used 3-4µm and prefer HIER over enzymes. René J. --- On Thu, 8/13/09, Katelin Lester <kate...@cuttingedgehistology.com> wrote:
From: Katelin Lester <kate...@cuttingedgehistology.com> Subject: [Histonet] optimal IHC thickness To: histonet@lists.utsouthwestern.edu Date: Thursday, August 13, 2009, 10:24 AM Hi everyone, I am staining lymph nodes for staging of breast cancer with CKAE1/AE3 and would like to know what everyone is cutting this type of slide at. I searched the archives and my understanding is that, for IHC, how thick the sections are cut is not necessarily critical. I typically cut all IHC at 4μm and I want to achieve the best results possible. Also, on a side note, the Data Sheet suggests using Trypsin, but I have been using Pepsin. Any comments or suggestions about time, quality of Trypsin vs. Pepsin? Thank you, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
