I use a dog chewed old stick. On another note, how to clean off dried mounting media. I have several objectives where some goombahs dragged the objectives through wet mounting media.
If y'all have any suggestions about removing dried mounting media. Send them my way. 4 of my 5 objectives are no longer objective after having their lenses clouded by the media. ;-) Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Fri, 8/21/09, Pamela Marcum <[email protected]> wrote: > From: Pamela Marcum <[email protected]> > Subject: RE: [Histonet] Cleaning oil off objectives > To: "'Rittman, Barry R'" <[email protected]>, > [email protected] > Date: Friday, August 21, 2009, 12:43 PM > Really like the taser idea! I > could have used that for several people over > the years. > > Pam Marcum > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] > On Behalf Of Rittman, > Barry R > Sent: Thursday, August 20, 2009 10:36 PM > To: [email protected] > Subject: RE: [Histonet] Cleaning oil off objectives > > Adam > Hi > I would strongly recommend that the best approach is to > train the people > using the microscope, everyone is trainable although for > some this may be a > long learning curve. > The use of a taser with the later individuals is strongly > recommended! > > Several years ago the Zeiss representative in Iowa used the > expanded plastic > packing beads to wipe off the excess oil as he said this > was much more > absorbent for oil that lens tissue. > We have also seen the use of soft wood tips with oil that > is encrusted on, > on the understanding that the wood is much softer than the > lens.. Never > completely happy with that concept. > A lot depends on the type of lens that is being used. > Some lenses, especially older ones may have a coating that > is easily damaged > even by Q tips. > I would use lens paper first (don't be cheap skate with the > lens tissue) > then repeat using a small amount of lens cleaner. The > most difficult and > usually the most contaminated seem to be the 40 due to its > working distance. > Most of the lens cleaners have isopropyl alcohol and some > acetone. > If it really does not get all the oil after repeating a > couple of times then > can use acetone but don't flood the lens just use small > amounts and wipe > across the face. Follow this with lens cleaner and lens > paper. > Has always worked for me. This sounds a lengthy procedure > but only takes a > couple of minutes. > Hope that this helps > Barry > > > ________________________________________ > From: [email protected] > [[email protected]] > On Behalf Of Adam . > [[email protected]] > Sent: Thursday, August 20, 2009 7:19 PM > To: [email protected] > Subject: [Histonet] Cleaning oil off objectives > > Hi all, > > You guys were so helpful on my last question, I'll ask > another. We have a > microscope shared by the floor with several objectives, and > it's pretty > common for the non-immersion objectives to get contaminated > with oil. I > asked the guy who is responsible for the scope about this. > He said that they > call someone from some company who carefully cleans the > objectives with > acetone and a Q-tip, which if done right works wonders but > if done wrong it > can damage the lenses. But he mentioned that the lenses are > usually > re-contaminated within a few weeks since so many people use > the scope, so > it's sort of a pointless endeavor. This system seems pretty > silly to me... I > feel like there must be an easier and cheaper way to clean > the lenses > without damaging them; I certainly don't want to be > responsible for damaging > a microscope that costs more than my yearly salary. What do > you recommend? > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
