Vinnie:
I always used the following solution:
Absolute ethanol -----------2000 mL
Distilled water ---------------- 680 mL
38% ("pure") formalin -------320 mL
acetic acid---------------------- 200 mL
The LN are revealed well
René J.
--- On Fri, 8/21/09, Della Speranza, Vinnie <del...@musc.edu> wrote:
From: Della Speranza, Vinnie <del...@musc.edu>
Subject: [Histonet] clearing agent for gross specimens
To: "Histonet" <histonet@lists.utsouthwestern.edu>
Date: Friday, August 21, 2009, 12:15 PM
I would appreciate hearing from anyone who routinely utilizes a clearing agent
in the gross room to clear fat from tissues to allow lymph nodes to be
visualized.
I am aware of Dissect Aid but would like to learn of other comparable products.
If individuals are using home made solutions are will to share their formula I
would appreciate receiving that information as well.
Our primary interest is speed of clearing for turnaround time reasons.
I am in the midst of a Histosearch search but it is slow going because most
discussions refer to tissue processing or staining so it's like looking for a
needle in a haystack
Thank you all
Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
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