We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1
hour followed by 10 minutes in 50% Ethanol rinsing in several changes of
buffer afterward and it seems to be working well for a multitude of
autofluorescent problems on FFPE sections for us. We tried to Sodium
Borohydride and it was a little diffiucult to work with....quite hazardous.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: port...@msu.edu
Web: www.humanpathology.msu.edu
----- Original Message -----
From: "Thurby, Christina" <christina.thu...@bms.com>
To: "Thurby, Christina" <christina.thu...@bms.com>;
<histonet@lists.utsouthwestern.edu>
Sent: Monday, August 24, 2009 11:00 AM
Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence
on FFPE sections for IF examination
Sorry I did not include my contact information.
Christina Thurby
christina.thu...@bms.com
812-429-8097
Thanks!
-----Original Message-----
From: Thurby, Christina
Sent: Monday, August 24, 2009 9:59 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: reduction/elimination of red cell autofluorescence on FFPE
sections for IF
examination
Can anyone give feedback on reagents/procedures to use for
immunofluorescence to
reduce/eliminate red cell autofluorescence on FFPE sections. I am using an
indirect method
of labeling for two antibodies (FITC and Texas Red).
I have not tried 0.1% sodium borohydride. Will this help for formalin
fixed specimens? I
have read that it is used for gluteraldehyde autofluorescence reduction.
If appropriate how
long should this reagent be applied to the specimen and at what
temperature.
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