Hi Maria, Are you saying you are using formalin as a preservative for non-gyn's? We collect all aspirations in Plasmalyte which is an electrolytic balance for infusion. All other fluids are collected in a green top, sodium heparinized tube. Bronch specimens are sent in saline. Urine and CSF are sent fresh. When a cell block is requested we centrifuge the sediment to get a button. Pour off the supernate. Then we use HistoGel to make the button. As indicated, HistoGel is a gel you heat up in the microwave. Pour off an equal amount into the non-gyn button let it solidify then you process the solid material just as any other specimen. Hope this helps.
Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net Maria Katleba <maria.katl...@stjoe.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 08/25/2009 01:00 PM To "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Trouble making a good Cell Block Hi All, Can anyone give me a good protocol for making a cell block from a non-gyn fluid? (abdominal fluid, bronchial wash, pleural fluid, etc) First of all, we have switched to formalin. Yes! Formalin!! So as you would expect, no button forms. The reason? The pathologists believe the formalin is better than alcohol (95%) especially when you expect to run IHCs on the cell blocks. Please send me ideas!!!! Maria Katleba HT(ASCP) MS Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet