Hello- Has anyone tried to counterstain an IHC stain with Luxol Fast Blue? I am worried that the LFB destain will remove some of the IHC.
I use the VIP solution from Vector as the chromogen for IHC instead of DAB and I destain Luxol Fast Blue with Hydroquinone/Sodium Sulfite (in addition to the ethanol steps). I'm staining for Ki-67 and I'm doing this on FFPE human brain sections. I haven't found anything in the Histonet archives on this topic. Any advice would be greatly appreciated. Thanks in advance! -Nicole, Post-bacccalaureate Fellow, NIH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
