Edie Lehman MT(ASCP), Anatomical Pathology Supervisor at Fairfield Medical Center in Lancaster, Ohio asks:
>>I am currently writing a procedure for the use of Toluidine Blue staining for >>both FNA's and frozen sections at the request of one of our pathologists. >>Can anyone guide me in the use of this stain in lieu of Diff-Quik or H&E for >>rapid processing (such as references I could use, etc) and does anyone have a >>procedure I can cite for permanent mounting/storage of these slides?<< If you want to prepare a suitably buffered solution of toluidine blue for frozen sections, you'll have to look up a formula. If you don't want to do this, write your procedure for Diff-Quik II (the blue solution) or one of a number of generic equivalents available. (Don't use a combined stain such as Sigma's for this purpose, unless you also want eosin.) Stain for a few seconds (slosh the slide through the stain till it's evenly wetted, dehydrate rapidly through alcohols, then into xylene (or substitute), mount in resin. Control slides are usually unnecessary, and are an impediment to getting the frozen section done rapidly. If the pathologist wants the eosin also (Diff-Quik I or generic equivalent), then follow the package insert. Diff-Quik (note the spelling) is a trademark of whatever Scientific Products is called this week. I would use one of the standard pathology books on frozen sections - your pathologist should have a copy - as a reference, just to have something on paper. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet