We use biotin blocking only with certain tissues, i.e. liver, kidney, GI, or diseases, i.e. oncocytomas.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Tuesday, September 01, 2009 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endogenous biotin blocking I recently had a discussion with one of my coworkers about the need/requirement for blocking of endoegnous biotin whenever an avidin-biotin detection system is used, and I was hoping that the IHC experts on the histonet might be able to provide us with some feeback. Its been my understanding that blocking is only necessary when one is certain that background staining is caused by endogenous biotin, but maybe I'm off-base here. I look forward to eveyone's input. Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet