Hi,

Can anyone out there recommend the best way to stain mouse mast cells in the 
kidney(toluidine blue/or berberine sulfate) with a protocol that produces 
cconsistent results?

Secondly, I have mouse kidney sections fixed in FFPE, PLP and methyl Carnoys. 
Does anyone know of a company that supplies antibodies  which will work on any 
of these fixatives,for the staining of the IgE receptor, mast cell trytptase 
and chymase.

Any advice would be appreciated!


Kim O'Sullivan
Monash University
Melbourne
Australia

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