Hi all, Just to update you, I ordered Abcam's ab13970 chicken anti-GFP and tested it out in paraffin embedded sections with HIER for 10 minutes in Dako target retrieval system. I came in with a Dylight 488 secondary from Jackson Immunoresearch at 1 ug / mL. It worked beautifully at 1:500, 1:1000, and 1:2000. I should've titered it even further down.
Thanks for all your help, Adam On Thu, Aug 20, 2009 at 1:38 PM, Hobbs, Carl <carl.ho...@kcl.ac.uk> wrote: > > I am sure someone has a cunning combination! > I use Abcam's ab13970 chicken anti GFP in Pwax sections after HIER. > For me, it is very good > Image at Abcam or here : http://www.immunoportal.com/index.php > Good luck! > carl > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet