Hello Histonetters.
I have recently stained for f4/80 macrophage marker on wax embedded mouse tibia sections. I have managed to get staining however, I also have staining on my negative control, which seems quite specific. I have investigated this and there seems to be no problems with the DAB and all reagents used are exactly the same down to where they are bought as those recommended in a protocol. If you have managed to stain for f4/80 on tibia sections without getting staining on your control or you have any explanations for this I would much appreciate hearing from you to compare protocols. Thanks Julia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet