Hello histonet! I'm trying to figure out the cause of some artifact in my ffpe h&e skin slides. Every once and a while I get a batch of slides with this strange separation artifact mostly around melanocytes. Its like there is a space around each cell(s). Also the collagen seems to have some odd stretching.
I tried recuting to make sure there was no microtomy issues but the artifact remains. I check my waterbath temp / soaked the blocks. Not soaked the blocks. I'm at a loss. This happens to entire processing batch. The doctors say they are still pathologically readable but I still want to find a way to fix this. Sent from my Verizon Wireless BlackBerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
