Hi Histonet! Regarding the discussion about the thickness of FFPE sections for Rhodanine copper, ie 3 um vs. 6 um, someone asked me: If everyone agreed that thicker sections are better, how it can be a 50/50 split about the thickness? Isn't that an unsustainable contradiction? (and I thank them for that question too!) I was saying that yes everyone agrees that more copper will show up in thicker sections and 50 % of the respondents said that they can demonstrate copper in thin sections too however the positive staining will be a little weaker than in the 6 micron sections. I hope that answers the question.
I'm the one who does not want to change our procedure until our lab can prove 3 um sections demonstrate copper. The problem is that we get unstained slides cut at 3 um and no block (consult cases). Also, someone asked if: "In this case, do thicker sections hold more intact cells?" because the " intact nuclei will hold more stain/dye than sectioned nuclei (not sealed to the slide) in a procedure that requires a 'differentiation' step with the mordant." I'm sure the answer to that is, "It depends." Thanks for all your help! Carrie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
