Hello Fellow Histonetters,

I have been trying to optimize CD3 from Abcam (ab56313) for quite awhile now 
and I am at my wits end with it. I have tried various retrieval methods and 
dilutions and polymer kits all to no avail. Has any researcher out there worked 
with this CD3 before or has a CD3 marker that they found to work and would you 
be willing to share what you did to get it to work.  I have this group 
breathing down my neck because they wanted their results by last week.  Any 
help would be greatly appreciated, thanks!!!

John


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