I am trying a protocol that uses 0.1M Tris/0.15M sodium chloride pH7.5, which is what I normally use. At another step in the protocol it says to wash with 0.05M Tris buffer pH 7.5. What do I use to make the 0.05M Tris buffer? Is it a combination of Tris HCl and Tris base without sodium chloride? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
