Hi, I have obtained fresh rat brains (not fixed) that have been directly snap-freezed in isopentane and stored at -80C. I plan to section 20um thick. I want to know if there is way to store the cut sections before I carry out the in situ hybridization without thawing and freezing? taht is, the cut sections will be frozen but when placed on slides, but they will thaw before I can store them in the fridge (either -20C or -80C) and then before conducting my experimemt i will have to thaw it again.
Thanks, Anand Vasudevan Nanyang Technological University Singapore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
