Perhaps the antigen is soluble and diffused out of the
tissue into the buffer?
Jerry Ricks
Research Scientist
University
of Washington
Department of Pathology
histonet@lists.utsouthwestern.edu
Perhaps reversal is not the right word...slides were left overnight in
buffer w/ tween. We got very minimal faint staining compared to previous
runs done in one day.
Brett
-----Original Message-----
From: Morken, Tim [mailto:timothy.mor...@ucsfmedctr.org]
Sent: Tuesday, October 20, 2009 2:29 PM
To: Connolly, Brett M; histonet@lists.utsouthwestern.edu
Subject: RE: Antigen retrieval question
Brett, how long? I've left them overnight before proceeding without any
problems.
It would not be "re-fixation" as with formalin. However, if it is in a
detergent buffer it might cause some other kind of denaturation.
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Tuesday, October 20, 2009 11:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antigen retrieval question
Has anyone experienced a "reversal" of citrate HEIR unmasking do to a
prolonged delay in continuing the experiment after the retrieval step?
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com
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