Hi, I am doing whole mount IHCs on embryonic organ cultures and imaging them on a confocal microscope. The IHCs are going well, but I’m having trouble making the tissue stay in a specific position for imaging. I have been putting the tissue in the wells of depression slides, adding about 30uL of Vectashield mounting medium, then coverslipping. This works fine, except that I have little control over the final position of the tissue—as I put the coverslip down, the tissue often moves. Any suggestions would be welcome! Is there, for example, some kind of optically neutral agarose that I could spot around the tissue before adding the Vectashield and coverslip?? Or—other ideas?? Thanks, Alice
Alice Fleming Department of Human Genetics University of California Los Angeles Los Angeles, CA 90095 310-267-2456 ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
