Couldn't agree with Gayle more! Glucose oxidase is the best protocol for blocking endogenous peroxidase but isn't entirely necessary unless you are working with hematopoietic tissue or tissue that has a large leukocyte infiltrate. For muscle standard 0.3% H202 should be fine. Of course if you happen to be staining for leukocytes (neutrophils) in your muscle section, I would just buy the reagents and do the glucose oxidase afterall. It's simply the best. That being said, I do muscle IHC on frozens for endothelial markers, and use 0.3% H2O2. I do see bubbling but my sections do not come off and morphology looks great at the end. I use plus slides and dry my slides for several hours before putting at -80 deg C. This is both with PFA fixed tissue and acetone. Good luck, Andrea
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