I am processing adult rat brain sections for routine histological stains, but
am having a terrible time getting them to stick to the slide. After
transcardial perfusion the rat brains are dissected out and processed for
frozen sectioning. I post-fix overnight in PFA, cryoprotect in 30%sucrose
until the brains sink and flash freeze in isopentane. All seems like pretty
standard stuff. After cutting 45 um thick sections and storing them in
antifreeze solution (30% 0.1M Phosphate Buffer, 30% Glycerol, 40% Ethylene
Glycol), I mount them on slides. After letting them dry overnight, I
rehydrate/wash in 0.1M Phosphate Buffer. Frequently, the sections start to
wrinkle up and start coming of the slide during this first step. I'm using
superfrost+ slides from fisher. Does anyone have any advice? Please help.
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