Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail
----- Original Message ---- From: Gudrun Lang <gu.l...@gmx.at> To: histonet@lists.utsouthwestern.edu Sent: Fri, November 20, 2009 2:16:49 PM Subject: [Histonet] rhodanin stain fading Hi! Yesterday I did a rhodine stain for copper. Immediatly after staining I saw not many but distinct red granula in hepatocytes. I am new to this stain, so I was happy (and a little bit proud), that it had worked. I also stained one slide over night for comparison. Today morning the first stained slides has faded and not the smallest bit of red colour could be seen. Is this a common problem? What causes the fast fading? Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
