Hi all, i just can not get this IBA1 (marker of microglia) well stained on my frozen sections fixed with acetone (sections were either dried up in air for 1 hour or fixed immediately). But I always get good staining on PFA-perfused brain tissue.
Will IBA1 antigen reactivity lose in acetone fixation? 2009-11-26 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet