Karie, Basic fuchsin works well for microcracks - stain before processing;cutting; grinding. Schedule used will depend in size of bone and species. You want to avoid over-staining. If time allows, check literature in: Journal of Orthopedic Research; Bone; and American Journal of Veterinary Research - 2001 - present, for starters. Feel free to contact me. VK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet