In the past, when we made up 1% Alcian blue in 3% acetic acid (using
bottle distilled water), the pH was always 2.5. We did not have to
modify it to get the proper pH. Still using the same powder, we are
getting a pH of 2.1. I hate to adjust it because it seems to affect the
staining. Any suggestions. The pH meter is calibrated monthly.
Diana
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