My teacher, long retired Miss Mavis Marie, a MT that loved Histology, taught us that if we needed to do the staining the next day, to leave them in 70% alcohol. We did find that sometimes, not always, if left in distilled H2O overnight, it would wash off, but never did when left in 70%.
Hope this helped, Lynette >>> Rene J Buesa <rjbu...@yahoo.com> 12/7/2009 9:24 AM >>> No, it is not a good idea. In many occasions leaving the dewaxed sections in DW for prolonged periods of time can cause the section to peel off (not always, but sometimes). The idea is to dewax and do the special stains immediately after. René J. --- On Mon, 12/7/09, Charles, Roger <rchar...@state.pa.us> wrote: From: Charles, Roger <rchar...@state.pa.us> Subject: [Histonet] slide handling for special stains To: "Histonet (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Date: Monday, December 7, 2009, 8:19 AM Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rchar...@state.pa.us<mailto:rchar...@state.pa.us> No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet